Lead development, Metabolic Activity and Toxicity testing
The pre-clinical chemical mimetic development focuses on a NRP molecule deriving from a large protein involved in vesicular protein excretion. - The shortest amino acid chain providing all the desired bioactivities (see background) is eleven amino acids long.
- Amino acid modification (chemical mimetic generation) has been performed to enhance the overall physico-chemical stability of the molecule.
- Currently, excellent stability over a timeframe of 1 year stored under subzero conditions has been achieved.
- Metabolic activity is excellent as confirmed by ADME analysis showing a much longer half-life in human liver cells than in rat cells.
Toxicity Testing
In-house performed long-lasting toxicity studies (30 days) using peripheral administration route of the lead compound (boosts once a day) revealed no toxicity even if 100 x times the dosage of the upper efficacy dose range was applied.
NRP treated rats performed in spatial memory tests as normal as saline-injected naïve controls.